index-celllabeling2007

There are important indications for performing imaging procedures with cell labeled radiopharmaceuticals. There are special handling and anticoagulation techniques required to achieve optimal labeling.

There are three methods of labeling Red Blood Cells-RBC with Tc-99m. All are simple, rapid, inexpensive and reliable. It is important to compare the advantages and disadvantages of each, and predict the labeling efficiencies obtained with each method.

	Invivo / Invivo
	Invivo / Invitro
	Invitro / Invitro

Other labeling protocols involve White Blood Cells, Cromium 51 and platelets.

Cr-51 RBC's

It is very easy to label red cells with Cr-51.

Isolation of RBCs is not required.

Tc-99m WBC's

In-111 WBC's

It is easy to label White Blood Cells- WBCs with either Tc-99m or In-111.

Both preparations involve isolation of the WBCs and incubation with a lipophilic intermediate to effect labeling.

In-111 platelets

There are special cases such as GI Bleeding studies or hemangioma localization. GI Bleeding studies are optimally performed with Tc-99m RBCs prepared using the packed cell technique or the UltraTag kit.

Most labeling procedures can be performed in any laboratory by a person knowledgeable about aseptic technique.

There are important indications for performing imaging procedures with cell labeled radiopharmaceuticals. There are special handling and anticoagulation techniques required to achieve optimal labeling.

There are three methods of labeling Red Blood Cells-RBC with Tc-99m. All are simple, rapid, inexpensive and reliable. It is important to compare the advantages and disadvantages of each, and predict the labeling efficiencies obtained with each method.

Invivo / Invivo

Invivo / Invitro

Invitro / Invitro